NAMCS: "Ultrabright Plasmonic-fluor as a Cross-platform Nanolabel for Femtomolar Detection of Bioanalytes”
All dates for this event occur in the past.
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The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging. Here, we show that a plasmonic nanoscale construct serving as an ‘add-on’ label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices. The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element. Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition. It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
To attend
Dates are for synchronous sessions, with live introductions and Q&A, will be held at 3:00 p.m. ET on Thursdays and 4:00 p.m. ET on Fridays via Zoom webinar. Links to asynchronous talks will become available at least one week before the scheduled seminar date. The asynchronous talks will be closed captioned.
The presentation link is available in Carmen.